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Evidence for the formation of a γ-phosphorylated glutamyl residue in the Escherichia coli acetate kinase reaction

✍ Scribed by John A. Todhunter; Daniel L. Purich


Book ID
118309148
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
462 KB
Volume
60
Category
Article
ISSN
0006-291X

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A continuous assay for an intracellular
✍ H.Michael Koplove; Charles L. Cooney 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 452 KB

A continuous, automated assay for the measurement of acetate kinase activity in Escherichia co/i is described. The assay system consists of three steps: continuous sonication, acetate kinase catalyzed production of ATP, and subsequent measurement of ATP concentration. Complete extraction of acetate