Evidence for (PNA)2/DNA triplex structure upon binding of PNA to dsDNA by strand displacement
✍ Scribed by Peter E. Nielsen; Michael Egholm; Ole Buchardt
- Book ID
- 102372382
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 412 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0952-3499
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The binding of PNA (peptide nucleic acid) T~2~CT~2~CT~4~‐LysNH~2~ to the double‐stranded DNA target 5′ ‐A~2~GA~2~GA~4~ was studied by KMnO~4~ and dimethylsulfate (DMS) probing. It is found that upon sequence‐specific strand displacement binding of the PNA to the dsDNA target concomitant protection of the N‐7 of guanines within the target takes place. It is furthermore shown that the binding of this PNA is more efficient at pH 5.5 that at pH 6.5 and very inefficient at pH 7.5. These results clearly indicate that C^+^G Hoogsteen base pairing is present and important for binding and that the strand displacement complex therefore involves a PNA·DNA‐PNA triplex.