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Evidence for p15 cleavage site in myc-specific proteins of avian MC29 and OK10 viruses

✍ Scribed by Vlasta Sovová; Miloslav Trávníček; Ivo Hložánek; Hana Černá; Kari Alitalo; Antti Vaheri


Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
415 KB
Volume
28
Category
Article
ISSN
0730-2312

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✦ Synopsis


Myc-related proteins were precipitated from MC29 virus-transformed cells (PR-2) and from OKlO virus-transformed cells (9C) by anti-gag and anti-myc sera. Immunoprecipitates were cleaved with the avian retroviral protease p15 and the cleavage products analyzed in SDS-PAGE. Cleavage fragments of pl 10&ag-myc (product of MC29 virus) and p5gmYc (product of OKlO virus) showed the presence of a p15 cleavage site within the myc-specific region. The site is missing in deletion mutants of MC29 virus.