Evidence for glycosylphosphatidylinositol anchoring of intralumenal alkaline phosphatase of the calf intestine
β Scribed by Eva HOFFMANN-BLUME; Marvin B. GARCIA MARENCO; Heidrun EHLE; Renate BUBLITZ; Margarete SCHULZE; Anton HORN
- Book ID
- 115128679
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 829 KB
- Volume
- 199
- Category
- Article
- ISSN
- 1432-1327
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## Abstract Surprisingly alkaline phosphatase (AP) (EC 3.1.3.1) of calf intestine is found in large amounts, e.g. 80%, within chyme. Most of the enzyme is present as a mixture of four differently hydrophobic anchorβbearing forms and only the minor part is present as an anchorless enzyme. To investi
## Abstract A modified and improved method for isolation of calf intestinal alkaline phosphatase is described. By this method 300 to 400 mg of pure enzyme was prepared in a relatively short time. On the basis of the results of ultracentrifugation and of the free, polyacrylamide and immunoelectropho