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Evidence for Disulfide Bonds in Membrane-Bound and Solubilized Opioid Receptors

✍ Scribed by Keita Kamikubo; Hiroshi Murase; Masanori Murayama; Masafumi Matsuda; Kiyoshi Miura

Book ID: 111174741
Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 657 KB
Volume: 50
Category: Article
ISSN: 0022-3042
DOI: 10.1111/j.1471-4159.1988.tb02940.x

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The mobility of purified p opioid binding protein in SDS-polyacrylamide gek electrophoresis is sensitive to the presence of reducing agents. In the presence of increasing concentrations of DTT the apparent molecular weight increases in a stepwise fashion from 53 kDa to 65 kDa. This reduction in mobi