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Evidence for a single pool of myo-inositol in hormone-responsive WRK-1 cells

✍ Scribed by Dr. Marie E. Monaco; Nancy H. Moldover

Book ID: 102878914
Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 619 KB
Volume: 59
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240590208

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✦ Synopsis

Previous reports have suggested the existence of at least two pools of cellular myo-inositol (Ins); it has been further hypothesized that only one of these pools is utilized during hormone-activated, cyclic phosphatidylinositol (Ptdlns) resynthesis. In an effort to investigate this possibility, we have undertaken kinetic studies of Ins metabolism in WRK-1 cells. Our results indicate that a single pool of Ins is involved in both basal and activated Ptdlns synthesis. Ins generated by the hydrolysis of phosphatidylinositol4,5-bisphosphate (PtdlnsP2) mixes with the existing pool of free Ins and is not used exclusively for resynthesis of Ptdlns. e 1995 WiIey-Liss, ~n c . *

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