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Evidence for a presynaptic blockage of transmission in a temperature-sensitive mutant ofDrosophila

โœ Scribed by Koenig, J. H. ;Ikeda, Kazuo

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
691 KB
Volume
14
Category
Article
ISSN
0022-3034

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โœฆ Synopsis

In the temperature sensitive mutant of Drosophila, shibireb' (shi), synaptic transmission in the dorsal longitudinal flight muscles (DLM) is normal at 19OC, but is diminished progressively as the temperature is raised, and is blocked at 29OC. The purpose of this paper is to determine whether this defect is located presynaptically, postsynaptically, or both. It is demonstrated here that the postsynaptic sensitivity to L-glutamate, the putative transmitter for this synapse, is not decreased at 29OC. Furthermore, studies conducted with genetic mosaics of this mutant show that transmission is blocked when a mutant motor neuron synapses on a wild-type muscle fiber, but is not blocked when a wild-type motor neuron synapses on a mutant muscle fiber. Thus, the shi phenotype (temperature dependent transmission block) correlates with a shi motor neuron, not with a shi muscle fiber. The data, therefore, suggest that the defect is not postsynaptic, but presynaptic.

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