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Evidence for a phosphohistidine protein intermediate in the phosphoglycerate mutase reaction

✍ Scribed by Zelda B. Rose

Book ID
115700621
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
506 KB
Volume
140
Category
Article
ISSN
0003-9861

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An excellent substrate of methylmalonyl-CoA mutase, methylmalonyl-carba-(dethia) coenzyme A (methylmalonyl-CH(2)-CoA), was synthesized by a chemoenzymatic method and its alpha-proton was exchanged with deuterium by long-term incubation in deuterium oxide at pH 6.9. After addition of highly purified