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EVIDENCE FOR A LOCALIZATION SIGNAL IN THE 3′UNTRANSLATED REGION OF MYOSIN HEAVY CHAIN MESSENGER RNA

✍ Scribed by JOHN W. WISEMAN; L.ANNE GLOVER; JOHN E. HESKETH

Publisher: Elsevier Science
Year: 1997
Tongue: English
Weight: 174 KB
Volume: 21
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.1997.0140

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✦ Synopsis

Localization signals in the 3'untranslated region (3'UTR) of myosin heavy chain mRNA were investigated using hybrid gene constructs. In myoblasts transfected with constructs containing either both coding sequences and 3'UTR of the rabbit beta-globin gene or the beta-globin coding sequences alone in situ hybridization showed that globin transcripts were distributed throughout the cytoplasm with no localization. In contrast, in myoblasts transfected with beta-globin coding sequences linked to the myosin heavy chain 3'UTR there was strong perinuclear localization of the hybrid mRNA; this was maintained in myotubes. We conclude that myosin heavy chain 3'UTR contains a localization signal.

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