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Evidence for a functional association of DNA synthesis with the membrane in mitochondria ofSaccharomyces cerevisiae

✍ Scribed by Ruth M. Hall; John S. Mattick; Sangkot Marzuki; Anthony W. Linnane

Publisher
Springer
Year
1975
Tongue
English
Weight
294 KB
Volume
2
Category
Article
ISSN
0301-4851

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✦ Synopsis

We have studied the effect of membrane fatty acid composition on replicative DNA synthetic activity in mitochondria isolated from Saccharomyces cerevisiae. Cells containing different levels of membrane unsaturated fatty acids were obtained by growth of a fatty acid desaturase mutant of Saccharomyces cerevisiae in glucose-limited chemostat cultures supplemented with various concentrations of Tween 80. Arrhenius plots of DNA synthetic activity in isolated mitochondria show a discrete discontinuity at specific temperature which are dependent on the membrane unsaturated fatty acid content of the mitochondria. This indicates a functional association of DNA replication with the mitochondrial membrane in Saccharomyces cerevisiae.

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