Evidence for a carbanion intermediate in catalysis by spinach ribulose-1,5-bisphosphate carboxylase/oxygenase
β Scribed by Anil S. Bhagwat; Bruce A. McFadden
- Book ID
- 115705921
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 546 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0003-9861
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In a preparation of soluble components from isolated spinach (Spinecia oleracea L.) chloroplasts, the activity of ribulose-l,5-bisphosphate carboxylase (EC 4.1.1.39) is strongly increased by 6-phosphogluconate or by NADPH at pH 8.0. When the thylakoid system is added to these soluble components (rec
The functions of H i s 291 , H i s 295 and H i s 324 at the active-site of recombinant A. nidulans ribulose-1,5-bisphosphate carboxylase/oxygenase have been explored by site-directed mutagenesis. Replacement of His 291 by K or R resulted in unassembled proteins, while its replacement by E, Q or N re