Evaluation ofin vitro stability of small unilamellar vesicles coated with collagen and chitosan
✍ Scribed by Shi, Xiang-Yang; Sun, Cao-Min; Wu, Shi-Kang
- Book ID
- 101227261
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 154 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0959-8103
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✦ Synopsis
To improve the in vitro stability of small unilamellar vesicles (SUV), the permeability of SUV, made from soyabean phosphatidylcholine (PC) and coated with collagen and chitosan, was studied using 5(6)-carboxyýuorescein (5(6)-CF) as a ýuorescence probe. The results showed that the coating with a collagen/PC weight ratio of 2, or a chitosan/PC weight ratio of 8, signiücantly decreased the permeability of liposomal membranes. In addition, the ýuorescence polarization method was used to study the inýuence of a coating with collagen and chitosan at the above ratios on the ýuidity of liposomal membranes, employing an intramolecular charge transfer compound, a 3-methoxy-4º-N,Ndimethylaminoýavone derivative (DMMF), as a ýuorescence probe. The ýuidity of a liposomal membrane coated with either of the two macromolecules showed no obvious changes, indicating that SUV coated with collagen and chitosan at appropriate weight ratios, can signiücantly improve the in vitro stability of liposomal membranes without disturbing their ýuidity.