Evaluation of Waters Silica Sep-Pak cartridges for short chain fatty acid ethyl ester recovery
✍ Scribed by Danielle Leblanc; André Morin; Alain Houde
- Book ID
- 101338298
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2000
- Tongue
- English
- Weight
- 81 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0268-2575
No coin nor oath required. For personal study only.
✦ Synopsis
Waters Silica Sep-Pak cartridges were evaluated for their performance in trapping short chain fatty acid ethyl esters. Liquid solutions of ethyl butyrate, ethyl valerate and ethyl caproate were injected onto cartridges. All the esters bound to the matrix and were eluted with four bed volumes of ethanol for ethyl butyrate and two bed volumes of ethanol for ethyl valerate and ethyl caproate. Samples of esters stripped with an air stream for 1 min and trapped on cartridges were found to vary by less than 8% for ethyl propionate, ethyl butyrate, ethyl valerate and ethyl caproate. Production of ethyl butyrate by Pseudomonas fragi CRDA 037 resting cells during biotransformation was ef®ciently evaluated using these cartridges. Variations between samples were found to be less than 10%.