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Evaluation of three different protocols of protein extraction for Arabidopsis thaliana leaf proteome analysis by two-dimensional electrophoresis

✍ Scribed by Ana M. Maldonado; Sira Echevarría-Zomeño; Sylvia Jean-Baptiste; Martha Hernández; Jesús V. Jorrín-Novo


Publisher
Elsevier
Year
2008
Tongue
English
Weight
646 KB
Volume
71
Category
Article
ISSN
1874-3919

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✍ Lucia Carboni; Chiara Piubelli; Pier Giorgio Righetti; Birger Jansson; Enrico Do 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 326 KB 👁 1 views

## Abstract The present study reports a comparison of recently described solubilizing methods, to set up a simple protocol for obtaining two‐dimensional (2‐D) gel electrophoresis maps of brain tissue. Different protocols were used for preparing rat brain homogenates and the resulting maps were comp