Evaluation of the Roche LightCycler parvovirus B19 quantification kit for the diagnosis of parvovirus B19 infections
โ Scribed by Sharleen Braham; Jayshree Gandhi; Stuart Beard; Bernard Cohen
- Book ID
- 116630565
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 143 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1386-6532
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The sensitivity and application of the polymerase chain reaction (PCR) for the diagnosis of parvovirus B19 (B19) infection was investigated by simultaneously assaying a collection of 279 consecutively received Samples for presence of anti-B19 IgM and IgG antibodies by Western blot and for B19 DNA by
Recombinant versions of parvovirus B19 capsid proteins VP1 and VP2 are used for immunodiagnostic assays for detection of antiviral antibodies. The immune response to B19 is characterized by a gradual loss of antibodies directed against linear epitopes of VP2. A similar occurrence for antibodies rais