Evaluation of the rat micronucleus test with bone marrow and peripheral blood: Summary of the 9th collaborative study by CSGMT/JEMS·MMS
✍ Scribed by Akihiro Wakata; Youichi Miyamae; Sei-ichi Sato; Takayoshi Suzuki; Takeshi Morita; Norihide Asano; Takumi Awogi; Koji Kondo; Makoto Hayashi
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 417 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
The mouse has traditionally been used for the micro-rat peripheral blood. Of the two mouse micronunucleus test, with bone marrow the usual target or-cleus-negative rat carcinogens, acrylonitrile was gan. The aim of the 9th collaborative study by positive in rat bone marrow and 4,4-methylene CSGMT was to evaluate the suitability of the rat bis(2-chloroaniline) was negative in both rat bone for the micronucleus test, with bone marrow and marrow and peripheral blood. Two chemicals reperipheral blood as the target organ. Since the rat ported to be mouse micronucleus-negative and ratspleen eliminates circulating micronucleated eryth-positive, azobenzene and Solvent Yellow 14, and rocytes, a rat peripheral blood micronucleus assay one chemical reported to be mouse-positive and ratmight not be feasible.
negative, 1,2-dimethylhydrazine, gave positive re-Thirty-four Japanese laboratories and six over-sponses in rat bone marrow and peripheral blood. seas laboratories participated in this collaboration, The concordance between bone marrow and peand 40 chemicals were studied. As a rule, rat bone ripheral blood with rats was 92%. The concordance marrow and peripheral blood were analyzed using between rat and mouse erythrocytes was 88%. We acridine orange staining.
concluded that the rat micronucleus assay, using Among 36 mouse micronucleus-positive rat car-either bone marrow or peripheral blood, can be cinogens, 34 of which had been evaluated by used as an alternative to the mouse micronucleus CSGMT, we observed 33 positive and three nega-assay. Environ. Mol. Mutagen. 32:84-100, tive results with rat bone marrow and 30 positive, 1998 ᭧ 1998 Wiley-Liss, Inc. three equivocal, and three negative responses with