A flow dialysis procedure with an approach to steady state in less than half a minute has been developed. The main advantage of the new device is that it permits the use of lower levels of radioactivity and therefore allows determination of dissociation constants (K,) of tight complexes, i.e., with
Evaluation of the Flow-Dialysis Technique for Analysis of Protein-Ligand Interactions: An Experimental and a Monte Carlo Study
✍ Scribed by Veldhuis, Gertjan; Vos, Erwin P.P.; Broos, Jaap; Poolman, Bert; Scheek, Ruud M.
- Book ID
- 118516053
- Publisher
- Biophysical Society
- Year
- 2004
- Tongue
- English
- Weight
- 215 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0006-3495
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