Evaluation of the ELISA test for detection of entamoeba histolytica in feces

✍ Scribed by Elizabeth Merino; Walter Glender; Ruben del Muro; Librado Ortiz-Ortiz

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 346 KB
• Volume: 4
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860040108

✦ Synopsis

The clinical utility of an ELISA test with tified. Of all patients, 11 6 were positive for the monoclonal antibodies to detect antigen of Entamoeba hisfolytica in feces was evaluated in 150 patients with gastrointestinal symptoms. Each subject was examined by rectosigmoidoscopy with rectal smear and/or a triple stool search for ova-bacteria-parasite (OBP); in addition, one stool sample was collected for the ELISA test. All the tests were independent and double blind. E. hisfolyfica was detected by OBP and/or rectosigmoidoscopy in 66 patients; 61 patients had other parasites; and in 23, no parasites were iden-ELISA test. Of these, E. histolytica was identified in 52. In 47, other parasites were identified and in 17, no parasites were found. The ELISA test with a monoclonal antibody against E. histolyfica antigen showed higher sensitivity than the standard diagnostic methods: the ability to detect the presence of E. his- rolytica antigen regardless of the destruction of the parasite or of the error due to misidentification of t h e parasite resulting from faulty preparation of the samples.