Evaluation of the detection of biomolecules in capillary electrophoresis by contactless conductivity measurement

✍ Scribed by Eva M. Abad-Villar; Pavel Kubán�; Peter C. Hauser

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 753 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200500495

✦ Synopsis

Evaluation of the detection of biomolecules in capillary electrophoresis by contactless conductivity measurement

The sensitivity of contactless conductivity detection to amino acids, peptides and proteins in CE was studied for BGE solutions of different pH values. The LOD and analytical characteristics were compared for acidic and basic conditions and better results were in most cases found for buffers of low pH values. Linear dynamic ranges varied between two orders of magnitude for amino acids and peptides and three orders of magnitude for larger proteins. The concentration detection limits were found to be between 1.2 and 7.5 lM for the amino acids tested and for the larger molecules they varied between 2.6 lM for leucine enkephalin and 0.2 lM for HSA when using a buffer at pH 2.1.