Evaluation of the cytotoxicity of calcium phosphate root canal sealers by MTT assay

✍ Scribed by Cüneyt Telli; Ahmet Serper; A. Lale Dogan; Dicle Guc

• Book ID: 117274786
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 352 KB
• Volume: 25
• Category: Article
• ISSN: 0099-2399
• DOI: 10.1016/s0099-2399(99)80303-3

✦ Synopsis

The purpose of this study was to evaluate the cytotoxicity of some calcium phosphate-based sealers (Sankin apatite root canal sealers (SARCS) types 1 to 3) in comparison with currently used sealers (CRCS, Ketac Endo, AH26, and Endomethasone) by using MTT assay on L929 cells. Monolayer cell cultures were prepared on 96-well plates. After incubation at 37 degrees C in a humidified 5% CO2-containing air atmosphere for 24 h in the presence of each sealer extracts, 25 microliters of 5 mg/ml of MTT in saline were added into each well and incubated a further 3 h at 37 degrees C. A solubilization buffer consisting of 23% sodium dodecyl sulfate in 50% N,N-dimethylformamide (pH 4.7) was used to dissolve formazan precipitate. The optical densities of the plates were then read by a microplate spectrophotometer at 570 nm. Greater magnitude of optical density due to intense blue coloring is regarded as showing a higher percentage of cell viability. Among the different types of sealers, SARCS types 1 to 3 and CRCS did not exert any cytotoxic effects, whereas AH26, Ketac Endo, and Endomethasone produced some cytotoxicity.