Evaluation of Teflon-coated intraocular lenses in an organ culture method

✍ Scribed by Werner, Liliana ;Legeais, Jean-Marc ;Nagel, Marie-Danielle ;Renard, Gilles

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 668 KB
• Volume: 46
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19990905)46:3<347::aid-jbm6>3.0.co;2-m

✦ Synopsis

An amorphous and transparent form of Teflon is proposed as a coating of polymethylmethacrylate (PMMA) intraocular lenses (IOLs), rendering them highly hydrophobic. We used an organ culture method to evaluate cell adhesion, proliferation, and migration on Teflon-coated IOLs. Corneal explants from 14-day-old chicken embryos were placed on a semisolid culture medium and covered with uncoated PMMA (n = 36) and Teflon-coated PMMA (n = 36) IOLs and two controls, Thermanox (n = 84) and latex (n = 36). After incubation (7 days at 37°C), a digital imaging system was used to measure the areas of the cell migration layers on the materials. The cells were then removed with tripsin-ethylenediaminetetraacetic acid and the cells detached at times up to 75 min were counted (Coulter Mul-tisizer System). The values were used to construct a cell disconnecting curve for each material. The areas of cell migration layers on uncoated and Teflon-coated IOLs were significantly different (p < .05). Cell disconnecting curves demonstrated that cells adhered less strongly to Teflon-coated IOLs than to the other materials. This organ culture method demonstrated that the coating of PMMA IOLs with Teflon AF is correlated with antiadhesive and antiproliferative properties.