Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat–rye addition lines
✍ Scribed by Martin Pfosser; Erwin Heberle-Bors; Amelia Amon; Thomas Lelley
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 617 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
Flow cytometric DNA analysis was used to study changes in nuclear DNA content induced by the addition of complete or telosomic rye chromosomes into the genome of common wheat (Triticum aestivum L.). The DNA content of each addition line was determined by comparison with an internal reference value and was expressed as a difference with respect to the original wheat parental line. A 1.84% difference in the DNA content could be detected. Nuclei were flow sorted and the presence of rye D N A analysis by flow cytometry is now widely used in various aspects of biological research and clinical diagnosis. Whereas in clinical diagnosis detection of aneuploidy is an important prognostic tool in a variety of malignant diseases (14,27), in plants flow cytometry finds large application to determine different ploidy levels. Applications include the screening of seed lots for triploid plants during hybrid seed production in sugar beets (6) or the screening of haploid plants obtained by anther and This work was supported by the Austrian Research Council, project Address reprint requests to M. Pfosser, Institute of Microbiology and no. W174.