Evaluation of selective detectors for the rapid and sensitive gas chromatographic assay of cytokinins, and application to the analysis of cytokinins in plant extracts

✍ Scribed by R. J. Whenham

• Publisher: Springer-Verlag
• Year: 1983
• Tongue: English
• Weight: 737 KB
• Volume: 157
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00396888

✦ Synopsis

The performances of four gas chromatographic detectors (nitrogen-phosphorus, flamephotometric, electron-capture and flame-ionisation) were compared for the analysis of permethylated cytokinins. The nitrogen-phosphorus and flame-photometric detectors (selective for nitrogen and sulphur-containing compounds respectively) gave greatly enhanced sensitivity and-or specificity compared with the flame-ionisation detector for a wide range of cytokinin types. The response of the electron-capture and flame-ionisation detectors was quantitatively similar. The improved performance of the nitrogen-phosphorus and flame-photometric detectors was used to develop rapid and simple purification techniques for the assay of zeatin and zeatin riboside in coconut milk; dihydrozeatin and dihydrozeatin riboside in bean leaves and cytokinin nucleosides in wheatgerm transfer RNA.