Evaluation of sample treatment procedures for the routine identification and determination at nanogram level ofO6-monoacetylmorphine in urine by capillary GC and dual NPD-FID
✍ Scribed by Vu-Duc, Trinh ;Vernay, André
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 427 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0935-6304
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Three methods were evaluated for the determination of O^6^‐monoacetylmorphine (6‐MAM), the specific metabolite of heroin in human urine, by GC‐NPD‐FID. The procedure devised for a hydrophobic cation exchange column showed superior performances over the liquid‐liquid extraction technique and solid phase extraction with C~18~. 6‐MAM can be analyzed in random urines at the 2 ng/mL concentration with classical equipment for the differentiation between heroin intake and opiates from various origins. The procedure was also applied to enzymatic and acid hydrolyzates of urines. The simultaneous presence of morphine and codeine are useful in the interpretation of results.