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Evaluation of RT-PCR and immunohistochemistry as tools for detection of enterovirus in the human pancreas and islets of Langerhans

โœ Scribed by Skog, Oskar; Ingvast, Sofie; Korsgren, Olle


Book ID
127121764
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
960 KB
Volume
61
Category
Article
ISSN
1386-6532

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## Abstract A twoโ€step reverse transcriptase TaqManโ„ข duplex PCR (RTโ€PCR) assay was developed using the ABI 7700 Sequence Detection System for the detection of enterovirus (EV) and parechovirus type 1 and 2 (PEV) RNA from samples of cerebrospinal fluid (CSF) and throat swabs. Using sequenceโ€specific