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Evaluation of relative DNA binding affinities of anthrapyrazoles by electrospray ionization mass spectrometry

✍ Scribed by Suncerae I. Smith; Lynn J. Guziec; Frank S. Guziec Jr; Brian B. Hasinoff; Jennifer S. Brodbelt

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
172 KB
Volume
42
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Binding interactions of a new series of anthrapyrazoles (APs) with DNA were evaluated by electrospray ionization mass spectrometry (ESI‐MS). Relative binding affinities were estimated from the ESI‐MS data based on the fraction of bound DNA for DNA/anthrapyrazole mixtures, and they show a correlation to the shift in melting point of the DNA measured from a previous study. Minimal sequence specificity was observed for the series of anthrapyrazoles. Upon collisionally activated dissociation of the duplex/anthrapyrazole complexes, typically ejection of the ligand was the dominant pathway for most of the complexes. However, for complexes containing AP2 or mitoxantrone, strand separation with the ligand remaining on one of the single strands was observed, indicative of a different binding mode or stronger binding. Copyright Β© 2007 John Wiley & Sons, Ltd.

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