Evaluation of quantity and staining pattern of human papillomavirus (HPV)-infected epithelial cells in thin-layer cervical specimens using optimized HPV-CARD assay
✍ Scribed by Algeciras-Schimnich, Alicia ;Policht, Frank ;Sitailo, Svetlana ;Song, Minghao ;Morrison, Larry ;Sokolova, Irina
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 498 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
Background:
Testing for human papillomavirus (hpv) is used in the triage of women with a cervical cytology of atypical squamous cells of undetermined significance (ascus). a fluorescent in situ hybridization assay was developed for the detection of hpv using the catalyzed receptor deposition technique (hpv-card). in this study, the utility of this assay was tested for the detection of hpv in liquid-based cervical cytology specimens.
Methods:
A total of 195 liquid-based cytology specimens were analyzed using the hpv-card assay. the results from the assay were compared with hpv polymerase chain reaction (pcr) and typing results. the number of hpv-infected cells and the staining pattern was correlated with the cytology classification.
Results:
A 91% concordance between hpv-card and pcr was observed for the detection of high-risk hpv viruses. a 78% concordance was observed for specimens that were negative for hpv. in ascus, low-grade squamous intraepithelial lesion (lsil), and high-grade squamous intraepithelial lesion (hsil) categories, the average number of hpv-positive cells per slide was 19 cells, 127 cells, and 450 cells, respectively. the number of cells with a punctate staining, suggestive of hpv integration, was 21% in ascus, 34% in lsil, and 46% in hsil specimens.
Conclusions:
The results of the current study indicate positive correlations between the severity of the disease and the increased overall quantity of hpv-positive epithelial cells in cervical cytology specimens and accumulation of cells with punctate staining suggestive of integrated hpv. in summary, the developed hpv-card assay was found to provide novel information regarding the proportion and staining pattern of hpv-infected epithelial cells in different cytologic categories of cervical specimens.