Evaluation of Protein Depletion Methods for the Analysis of Total-, Phospho- and Glycoproteins in Lumbar Cerebrospinal Fluid
β Scribed by Ogata, Yuko; Charlesworth, M. Cristine; Muddiman, David C.
- Book ID
- 124051305
- Publisher
- American Chemical Society
- Year
- 2005
- Tongue
- English
- Weight
- 463 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1535-3893
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A procedure using high-voltage isoelectric focusing (IF) in ultrathin (02. mm) gels and enzyme-amplified immuno-sandwich assay was elaborated to get optimal IF separation conditions, to avoid CSF concentration, e.g. by ultrafiltration preceding IF with the risk of unequal protein losses, to minimize
## Abstract A biuret automated colorimetric assay for total protein in urine and cerebrospinal fluids was established. The procedures were as follows. Acidify all urine sample before analysis. Add precipitant Na~2~WO~4~ to urine samples. After 10 min, centrifuge, decant the supernatant fluid, drain