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Evaluation of PCR-based methods for the diagnosis of infection in bancroftian filariasis

✍ Scribed by Senarath Dissanayake; Abraham Rocha; Joaquim Noroes; Zulma Medeiros; Gerusa Dreyer; Willy F. Piessens

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
700 KB
Volume
94
Category
Article
ISSN
0035-9203

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✦ Synopsis

The value of the polymerase chain reaction (PCR) in the diagnosis of Wuchereria bancrofti infection was evaluated in comparison to microscopical examination of night blood smears, Nuclepore filtration, serology and ultrasonography. No correlation was found between PCR-based deoxyribonucleic acid (DNA) probing and serology. We did not find any evidence of free filarial DNA in either blood plasma or chylocoele fluid. We conclude that the 2 PCR-based techniques evaluated are not more sensitive than Nuclepore filtration for detection of W. bancrofti microfilaraemia, need at least 1 intact microfilaria in the volume of blood used for DNA extraction, and were much inferior to ultrasonography for detection of amicrofilaraemic adult worm carriers.

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