Evaluation of new supports for high-pressure aqueous gel permeation chromatography: TSK-GEL SW type columns
โ Scribed by K. Fukano; K. Komiya; H. Sasaki; T. Hashimoto
- Book ID
- 104143972
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 523 KB
- Volume
- 166
- Category
- Article
- ISSN
- 1873-3778
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โฆ Synopsis
New chemically modified silica gel based supports, TSK-GEL SW type columns, for aqueous gel permeation chromatography have been evaluated'in detail. Adsorption or denaturation of proteins and enzymes on these supports is negligible, the recoveries of the proteins and enzyme activities being both almost quantitative_ The applications of these supports for the study of proteins, enzymes, saccharides and water-soluble synthetic polymer have been investigated.
๐ SIMILAR VOLUMES
Trifluoroacetic acid (0.1% w/v) is an excellent solvent for polypeptides, is volatile, and has a low absorbance of ultraviolet light of low wavelength. Polypeptides subjected to chromatography on columns of TSK-G2000-SW or TSK-G3000-SW in this solvent were eluted as sharp peaks. Retention volume was