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Evaluation of new supports for high-pressure aqueous gel permeation chromatography: TSK-GEL SW type columns

โœ Scribed by K. Fukano; K. Komiya; H. Sasaki; T. Hashimoto


Book ID
104143972
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
523 KB
Volume
166
Category
Article
ISSN
1873-3778

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โœฆ Synopsis


New chemically modified silica gel based supports, TSK-GEL SW type columns, for aqueous gel permeation chromatography have been evaluated'in detail. Adsorption or denaturation of proteins and enzymes on these supports is negligible, the recoveries of the proteins and enzyme activities being both almost quantitative_ The applications of these supports for the study of proteins, enzymes, saccharides and water-soluble synthetic polymer have been investigated.


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High-performance gel permeation chromato
โœ G.Brent Irvine; Christopher Shaw ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 631 KB

Trifluoroacetic acid (0.1% w/v) is an excellent solvent for polypeptides, is volatile, and has a low absorbance of ultraviolet light of low wavelength. Polypeptides subjected to chromatography on columns of TSK-G2000-SW or TSK-G3000-SW in this solvent were eluted as sharp peaks. Retention volume was