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Evaluation of methods to extract and quantify lipids from Synechocystis PCC 6803

✍ Scribed by Jie Sheng; Raveender Vannela; Bruce E. Rittmann


Book ID
108070254
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
309 KB
Volume
102
Category
Article
ISSN
0960-8524

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A putative 4-alpha-glucanotransferase (alphaGTase) gene from Synechocystis sp. PCC 6803 was identified being composed of 1505 nucleotides, and the overexpressed protein was purified with an affinity chromatography. The recombinant alphaGTase had about 57kDa of molecular mass when judged by SDS-PAGE