β Scribed by Narasimha M. Rao; Kevin Hool; Timothy A. Nieman
No coin nor oath required. For personal study only.
Several sohd supports and munobkatlon procedures are evaluated for use m contammg lummol on pmcles m flow-through columns for use m determmatlon of hydrogen peronde Covalent attachment (via glutamldehyde and an ammoall&dane)
on slhca and on Ambersorb, adsorption on Ambersorb, and amon-exchange on a resm are considered Amounts of lummol bound (pmole g-l support) are 29 for adsorption on Ambersorb, 82 for covalent attachment, and 875 for Ion-exchange These amounts are sufficient for hundreds of determmatlons mth a smgle column With the covalent and adsorption unmobtiatlons, lummol1s released mto the flow stream by mJectlon of an alkalme sample This injected solution controls the flow stream pH mth Ambersorb, but sfica consumes hydroxide and leads to altered pH and degraded analytical performance With Ion-exchange munobdlzatlon the amomc concentration of the mjected sample determmes the amount of lurmnol released as well as the pH downstream of the column The resultmg hydrogen peroxide detection hnuts are 0 15 PM for adsorptlon, l-2 PM for covalent, and 5-10 PM for ion-exchange unmobdlzatlon The near-neutral pH used wth the Ion-exchange nnmoblhzatlon I compatible v&h enxyme reactions, and determmation of glucose IS demonstrated m a smgle channel flow system usmg munoblllzed hnnmol, munobtied HRP, and munobdlzed glucose oxldase High and vanable sample lomc strengths can be dealt wrath (for the Ion-exchange munobdlzatlon) by use of an ion-replacement sample pretreatment method Kizywork Chemdummescence, Plow metion, Immobdlzed reagents, Lummol The nnmoblllzatlon of analytlcal reagents to sohd supports has drawn attention m recent years for use m flow systems and m sensors Chenulummescence (CL) reagents have been the SubJect of some of this nnmoblllzatlon mterest, and reports of nnmoblllzed or sohd-state lummol [l-6], peroxyoxalates [7-91, and t&2,2'-brpyndmejruthemum(I1)
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