Evaluation of loop and pump injection systems in preparative liquid chromatography

✍ Scribed by Gazda, Krystyna ;Kowalczyk, Jerzy S.

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 416 KB
• Volume: 6
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240060508

✦ Synopsis

Abstract

The usefulness of pump or loop injectors in preparative liquid chromatography was evaluated. The concentration distribution of samples flowing through a UV detector cell, directly connected to the injector, was recorded at various flow velocities, at various diameters of loop tubing, and using various methods of injection [injection with sample volumes equal to a fraction or total loop volume, etc.]. The most advantageous methods were found to be either use of a loop injector to inject only a fraction of its total volume or use of a pump. Both of these methods ensure a almost rectangular concentration distribution.