Evaluation of lipoprotein size–density distributions from sedimentation coefficient distributions obtained at several solvent densities. I. Theory
✍ Scribed by J. L. Oncley
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1969
- Tongue
- English
- Weight
- 715 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
A method is presented for the treatment of distribution functions obtained by ultracentrifugal sedimentation velocity experiments on the plasma lipoproteins, so that adequate corrections can be made for the effects of diffusion and concentration dependence of the sedimentation coefficient. The method involves the use of modified equations derived from those presented in 1952 by Gosting for the transform from g*(S) to g(S) distributions. The evaluation of the molecular parameters of lipoprotein density and size, from a set of g(Sio) distribution functions obtained in solvents of varying density, is also outlined. Preparations of the p-lipoprotein of human plasma are found to follow a bivariate normal distribution that requires evaluation of five parameters, 31, u,, 22, 02 and p . The first two represent the mean and standard deviation of the lipoprotein density, the third and fourth the same quantities for the size, expressed as a "logarithmic diameter parameter", and the final value is a measure of the correlation between these two variables.