Evaluation of intrarenal oxygenation in mice by BOLD MRI on a 3.0T human whole-body scanner

✍ Scribed by Lu-Ping Li; Lin Ji; Scott Lindsay; Pottumarthi V. Prasad

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 495 KB
• Volume: 25
• Category: Article
• ISSN: 1053-1807
• DOI: 10.1002/jmri.20841

✦ Synopsis

Abstract

Purpose

To extend observations on intra‐renal oxygenation with blood oxygen level‐dependent (BOLD) MRI in human and rats to mouse kidneys imaged with a human whole‐body scanner.

Materials and Methods

Renal BOLD MRI studies were performed on a 3.0T scanner using a multiple gradient‐echo (mGRE) sequence with a custom‐designed 2.0‐cm surface coil to acquire six T~2~*‐weighted images in mice (N = 8) at an in‐plane resolution of 156 × 156 μm^2^. BOLD MRI data were obtained before and after administration of furosemide (10 mg/kg intravenously [i.v.]).

Results

The mean weight of eight mice was 24.6 ± 1.0 g. The baseline renal R~2~* (mean ± standard error [SE]) was 28.6 ± 2.1 seconds^–1^ in the renal cortex (CO), 35.4 ± 2.2 in the outer medulla (OM), and 21.2 ± 2.1 seconds^–1^ in the inner medulla (IM). The BOLD response to furosemide (ΔR~2~*) was 4.1 ± 1.4 in the CO, 10.1 ± 2.1 seconds^–1^ in the OM, and 3.4 ± 0.8 seconds^–1^ in the IM in mice.

Conclusion

Intrarenal BOLD MR images with sufficiently high resolution can be obtained on a human whole‐body scanner when combined with a small receiver coil to allow studies in mice. Both baseline R~2~* and ΔR~2~* values following administration of furosemide were consistent with previous experience in humans and rats. J. Magn. Reson. Imaging 2007. © 2007 Wiley‐Liss, Inc.