Whole-genome DNA amplification by multiple displacement (MD-WGA) is a promising tool to obtain sufficient DNA amounts from samples of limited quantity. Using Affymetrix' GeneChip Human Mapping 10K Arrays, we investigated the accuracy and allele amplification bias in DNA samples subjected to MD-WGA.
Evaluation of genome coverage and fidelity of multiple displacement amplification from single cells by SNP array
β Scribed by Ling, J.; Zhuang, G.; Tazon-Vega, B.; Zhang, C.; Cao, B.; Rosenwaks, Z.; Xu, K.
- Book ID
- 126820211
- Publisher
- Oxford University Press
- Year
- 2009
- Tongue
- English
- Weight
- 423 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1360-9947
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## Communicated by Ulf Landegren The scarcity of genomic DNA can be a limiting factor in some fields of genetic research. One of the methods developed to overcome this difficulty is whole genome amplification (WGA). Recently, multiple displacement amplification (MDA) has proved very efficient in t
## Background Whole genome amplification (WGA) and laser assisted micro-dissection represent two recently developed technologies that can greatly advance biological and medical research. WGA allows the analysis of multiple genomic loci from a single genome and has been performed on single cells fro