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Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration

✍ Scribed by Yan-qing Yuwen; Zi-mei Dong; Qing-hua Wang; Xiao-juan Sun; Chang-ying Shi; Guang-wen Chen


Publisher
Springer
Year
2010
Tongue
English
Weight
250 KB
Volume
38
Category
Article
ISSN
0301-4851

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Real-time RT-PCR method was exploited to identify endogenous reference genes in differentiating human T helper cells. When using this technology in our experimental system, finding a set of genes whose mRNA expression levels would not change appeared to be very challenging. Our initial plan to use t