Evaluation of encapsulated Newcastle disease virus liposomes using various phospholipids administered to improve chicken humoral immunity
✍ Scribed by Li-Ping Tseng; Chwei-Jang Chiou; Ming-Chung Deng; Mei-Hsiu Lin; Ryh-Nan Pan; Yi-You Huang; Der-Zen Liu
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 91 KB
- Volume
- 91B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
We propose the adjuvant effects of phospholipid liposome compositions using intranasal inoculation of a liposomal‐Newcastle disease virus (NDV) vaccine in chickens. The immunogenicity of three liposome formulations was determined in chickens using the hemagglutination‐inhibition (HI) test, nasal secretory immunoglobulin A and serum immunoglobulin A (IgG) antibody titers using the enzyme‐linked immunosorbent assay. The immune response against NDV antigens was determined after immunization with neutral charged liposomes composed of egg phosphatidylcholine (EPC) (60 μmol), cholesterol (Chol) (15 μmol), and EPC‐liposomes (EPC‐Lip), which elicited strong systemic (serum) and local (nasal) humoral responses. However, the intranasal administration with cationic charged liposomes composed of EPC (30 μmol), stearylamine (SA) (15 μmol), Chol (15 μmol), and SA‐liposomes (SA‐Lip) induced poor humoral immune responses. Only the vaccine formulated with anionic charged liposomes composed of EPC (30 μmol), dipalmitoylphosphatidylserine (15 μmol), Chol (15 μmol), and phosphatidylserine‐liposomes (PS‐Lip) elicited the highest titers of HI antibodies. These are the first results to suggest that antigen delivery using EPC‐Lip is very useful in enhancing antibody production at the mucosal site and in serum. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009