Evaluation of densitometric quantitation of ether phospholipids by high performance thin layer chromatography
β Scribed by I. Coene; M. E. Ramos; E. Van den Eeckhout; P. Sandra; W. Van den Bossche
- Book ID
- 112733211
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 698 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0009-5893
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π SIMILAR VOLUMES
A method for the quantitation of small amounts of phospholipids derived from biological sources is described. Total phospholipid is determined by mineralization followed by the estimation of liberated phosphate by means of malachite green. The main phospholipid species are separated by one-dimension
The common mobile phase hexane/isopropanol/water used for separation of phospholipids on highperformance liquid chromatography silica columns poses several problems, such as incomplete separation and rapid column deterioration. By inclusion of 5 mM ammonium sulfate in the aqueous phase, we were able