Evaluation of dead time calculation in liquid chromatography using a linearization procedure for homologous series

✍ Scribed by van Tulder, P. J. M. ;Franke, J. P. ;de Zeeuw, R. A.

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 769 KB
• Volume: 10
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240100407

✦ Synopsis

A mathematical method for the calculation of the dead time (tM) in HPLC was evaluated using a computer simulationapproach, in which artificial perturbations were introduced to simulated homolog retention times. The calculation was based on a modified and extended Grobler and Balisz (GB) method. Investigated was how the precision of the calculated tM is affected by: statistical fluctuations in retention timesand which, and how many homolog retention times are used. Based on these simulations a two-step procedure for the tM calculation is proposed: In the first step the linearity of log tR,, vs carbon number n is checked using as many homolog retention times as possible. The slope value bo of the first linear regression in the GB method is used for the selection of homolog retention times in the final tM calculation. In the second step the optimal selection of homologs is made and the final ty calculation is carried out. Guidelines for homolog selection are given.