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Evaluation of core–shell particle columns for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides

✍ Scribed by Mirlinda Biba; Christopher J. Welch; Joe P. Foley; Bing Mao; Enrique Vazquez; Rebecca A. Arvary

Book ID
118477757
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
472 KB
Volume
72
Category
Article
ISSN
0731-7085

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The ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) of fluorescent-labeled oligonucleotides separation was established, and the concentration of triethylamine-acetic acid (TEAA, 0-0.15 M), pH value (4.5-7.0), and gradient strength were optimized. The retention mechanism o