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Evaluation of Biotin-OSu and Biotin-ONp in the Solid Phase Biotinylation of Peptides

✍ Scribed by Bodo Baumeister; J. Beythien; J. Ryf; P. Schneeberger; Peter D. White

Book ID
106452023
Publisher
Springer Netherlands
Year
2005
Tongue
English
Weight
196 KB
Volume
11
Category
Article
ISSN
1573-3149

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A competitive binding assay for biotin, biocytin, and desthiobiotin utilizing a genetically engineered enzyme-ligand conjugate is described herein. This assay is unique in that the enzyme-ligand conjugate consists of the streptavidin binding peptide Strep-tag II, which mimics the binding of biotin t