Evaluation of an Automated Equilibration Technique for Deuterium/Hydrogen Isotope Ratio Measurements with Respect to Assessing Total Energy Expenditure by the Doubly Labelled Water Method
✍ Scribed by Thielecke, F.; Noack, R.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 220 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
The doubly labelled water (DLW) method for measuring energy expenditure in free living subjects su †ers from inaccuracy and imprecision mainly owing to analytical problems. An automated H/D/O equilibration device was tested for precision, internal validity and matrix e †ects with particular consideration of DLW studies. The precision was found to be and for 2H and 18O, respectively. Dilution series with water standards and urine 0.5& 0.04& showed a high linearity and no matrix e †ects. In addition, the automated preparation is less laborious and time consuming and reduces sources of analytical imprecisions in studies measuring energy expenditure by the DLW method.