Evaluation of a spatial scanning laser fluorometric detector for capillary electrophoresis: Application to studies of band migration and dispersion
✍ Scribed by Brian K. Clark; Michael J. Sepaniak
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 759 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
Abstract
We report herein the development and evaluation of modified capillary electrophoresis instrumentation with laser fluorometric detection which allows for the convenient and precise translation of the detection zone along the length of the separation column. This instrumentation is utilized for fundamental studies of band dispersion, analyte mobility alterations, sample stacking, and the determination of diffusion coefficients. The feasibility of using this instrumentation to improve the signal/noise ratio and decrease analysis time is also presented. DNA restriction digest separations employing soluble polymer buffer systems with oncolumn fluorescent labeling was used to illustrate the unique advantages of this experimental design.