Evaluation of a retention model for high-performance ion-exchange chromatography using two different displacing salts

✍ Scribed by Mary Ann Rounds; Fred E. Regnier

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 616 KB
• Volume: 283
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96240-x

✦ Synopsis

A model proposed for the retention of proteins on high-performance ion-exchange chromatographic supports was further investigated via the use of NaCl vs. MgC12 as the chromatographic displacing salt. Evaluations of the Z term generated by this model were carried out using selected proteins on a strong anion-exchange support in an effort to define its meaning more clearly.

A positive correlation between Z and protein retention was found. Higher values for ZMsc,, were attributed to enhanced ionization of protein functional groups by Mg'+. Findings suggest that Z could be more precisely defined as the number of electrostatic interactions between the protein and the ion-exchange support.