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Evaluation of a photolabile derivative of 1,25-dihydroxyvitamin D3 as a photoaffinity probe for 1,25-dihydroxyvitamin-D3 receptor in chick intestinal cytosol

โœ Scribed by Rahul Ray; Stanley Rose; Sally Ann Holick; Michael F. Holick

Book ID
115758659
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
381 KB
Volume
132
Category
Article
ISSN
0006-291X

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โœ W.E. Duncan; T.C. Aw; P.G. Walsh; J.G. Haddad ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 460 KB

Cytosol prepared from small intestine of vitamin D-sufficient rabbits contains a specific highaffinity binding protein for 1,25dihydroxyvitamin D, (1,2S(OH),Ds). This binding protein sediments at 3.0-3.5 S in sucrose. density gradients containing 0.3 M KCI. Scatchard analysis using intestinal cytoso