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Evaluation of a peptide-based enzyme immunoassay for anti-SARS coronavirus IgG antibody

✍ Scribed by Paul K.S. Chan; W.K. To; Esther Y.M. Liu; T.K. Ng; John S. Tam; Joseph J.Y. Sung; Jean-Michel Lacroix; Michel Houde

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
70 KB
Volume
74
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

High throughput assays for anti‐SARS‐CoV IgG antibody detection are need for large‐scale epidemiologic studies. The performance of a microplate enzyme immunoassay, DETECT‐SARS™, was evaluated for the detection of anti‐SARS‐CoV IgG antibody. This assay is based on synthetic peptides derived from the nucleocapsid and spike proteins. The results showed that the assay provided a high degree of sensitivity (95.9%) for convalescent serum samples. The level of specificity was close to 90%, and did not show significant variation among different control groups. The high degree of sensitivity together with the high‐throughput nature makes it advantageous as a screening assay for studies where handling of a large number of specimens is required. J. Med. Virol. 74:517–520, 2004. © 2004 Wiley‐Liss, Inc.

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