Evaluation of a new matrix-free laser desorption/ionization method through statistic studies: comparison of the DIAMS (desorption/ionization on self-assembled monolayer surface) method with the MALDI and TGFA-LDI techniques
✍ Scribed by Matthieu Bounichou; Lionel Sanguinet; Kamal Elouarzaki; Olivier Alévêque; Marylène Dias; Eric Levillain; David Rondeau
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 273 KB
- Volume
- 43
- Category
- Article
- ISSN
- 1076-5174
- DOI
- 10.1002/jms.1414
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✦ Synopsis
Abstract
This work demonstrates that the desorption/ionization on self‐assembled monolayer surface (DIAMS) mass spectrometry, a recent matrix‐free laser desorption/ionization (LDI) method based on an organic target plate, is as statistically repeatable and reproducible as matrix assisted laser desorption ionization (MALDI) and thin gold film‐assisted laser desorption/ionization (TGFA‐LDI) mass spectrometries. On lipophilic DIAMS of target plates with a mixture of glycerides, repeatability/reproducibility has been estimated at 15 and 30% and the relative detection limit has been evaluated at 0.3 and 3 pmol, with and without NaI respectively. Salicylic acid and its d~6~‐isomer analysis confirm the applicability of the DIAMS method in the detection of compounds of low molecular weight. Copyright © 2008 John Wiley & Sons, Ltd.