Evaluation of a new disposable screen-printed sensor strip for the measurement of NADH and its modification to produce a lactate biosensor employing microliter volumes
✍ Scribed by Steven D. Sprules; John P. Hart; Robin Pittson; Stephen A. Wring
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 465 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1040-0397
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A combined sensor strip was fabricated by screen‐printing a Meldola Blue modified carbon working electrode and a Ag/AgCl combined reference/counter electrode alongside each other on a PVC substrate. This was investigated as an amperometric sensor for NADH, at an applied potential of 0 V, both in bulk solutions under hydrodynamic conditions and using 20 μL volumes without agitation. The former technique gave linear responses in the range 3–60 × 10^−6^mol dm^−3^ and the latter in the range 10−5 × 10^−4^ mol dm^−3^ of NADH. Further modification of the electrodes with NAD^+^ and lactate dehydrogenase converted the device to a biosensor for lactate both in spiked phosphate buffer and bovine serum. The sensors demonstrated good linear responses in the range 0–20 × 10^−3^ mol dm^−3^.