Evaluation of a catalase inhibitor in the developmental regulation of maize catalase

✍ Scribed by Tsaftaris, Athanasios S. ;Sorenson, John C.

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 746 KB
• Volume: 1
• Category: Article
• ISSN: 0192-253X
• DOI: 10.1002/dvg.1020010308

✦ Synopsis

A protein which has been shown to inhibit catalase in vitro appears to vary inversely with catalase activity in the maize scutellum during early sporophytic development when assayed using a catalase inhibition assay. This result suggested that the inhibitor protein may play a direct role in regulating catalase activity during this time period.

Four experimental approaches were used to evaluate this putative regulatory role, including immunological quantitation of individual catalase isozymes during germination using rocket immunoelectrophoresis, perturbation of normal catalase expression with hydrogen peroxide or allylisopropylacetamide (AIA), examination of a mutant line with an altered catalase developmental program, and direct radioimmunoassay of the inhibitor protein during germination. The results of these experiments indicate that the quantitative changes in catalase activity during development are not mainly due to changes in the expression of the catalase inhibitor. Other possible roles of this protein in catalase regulation are discussed.